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. 2021 Jul 16;12:4358. doi: 10.1038/s41467-021-24575-x

Fig. 1. Identification and characterization of novel readthrough agents using an RT/NMD NanoLuc reporter in FRT cells.

Fig. 1

a The reporter used to perform HTS. b Scatter plot of HTS results using the reporter cell line. The screen was comprised of 29 test sets. Hit compounds were defined as those with activity ≥ mean ± 3 SD of all test compound data (13.57%). Six data points are off-scale (460–2900%) and are not shown. Concentration-response data for c SRI-37240 alone (mean of 2 replicates, bars indicate range) and d comparison of SRI-37240 alone and combined with 100 μg/mL G418 in FRT cells expressing the NanoLuc RT/NMD reporter. In panel c, the NanoLuc activity is expressed as the percent (mean ± SD) of the signal generated by the positive control (300 μg/mL G418). In panel d, the NanoLuc activity is normalized to total cellular protein. Two-way ANOVA was used to compare NanoLuc activity (mean +/- SD) in cells treated with both SRI-37240 and G418 to cells treated with SRI-37240 alone (n = 4; *p < 0.05 and ****p < 0.0001). Source data is available as a source data file.