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. 2021 Jul 16;12:4358. doi: 10.1038/s41467-021-24575-x

Fig. 4. SRI-41315, a structural analog of SRI-37240, showed improved potency and efficacy in FRT cells that translated to 16HBE14o- cells.

Fig. 4

a SRI-37240 structure. b SRI-41315 structure. The average fold-increase in relative luminescence unit (RLUs) in c FRT cells or d 16HBE14o- cells stably expressing the W134X NanoLuc RT/NMD reporter after 24-h (FRTs) or 48-h treatment (16HBEs) of treatment with increasing concentrations (in µM) of SRI-37240 or SRI-41315. Average RLUs from 3 or 4 independent replicates were normalized to the average RLUs from cells treated with vehicle alone (0.3% DMSO). e NanoLuc RLUs (normalized to total lysate protein) from 16HBE14o- cells expressing the W134X NanoLuc RT/NMD reporter that were treated with SRI-37240 and SRI-41315 alone (at various concentrations shown in µM) alone or in combination with G418 (50 µg/mL). Each column indicates the mean+/−SD (n = 3 or 4). Exact p values (shown above the columns) were determined by a two-way ANOVA by comparing each treatment group to the untreated control with each cohort. All of the assays were independently repeated with similar results. Source data is available as a source data file.