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. 2021 Jul 16;11:14570. doi: 10.1038/s41598-021-93951-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a,b) Representative images of (a) non-decellularized and (b) decellularized hemi-palates. Each palate was washed in ultrapure water, treated in 4% sodium deoxycholate and then with DNase-I to be finally rinsed in ultrapure water. In order to remove decellularization reagents, samples were then washed with increasing percentages of denatured ethanol and finally rehydrated in ultrapure water. The macroscopic examination revealed that the color of the decellularized samples turned to white, while the non-decellularized ones showed a typical light pink shade.