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. 2021 Jul 16;11:14570. doi: 10.1038/s41598-021-93951-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a–l) Light and fluorescence microscopy images of non-pored (a–f) and pored (g–l) scaffolds stained either with HE (a,c,e,g,i,k) or DAPI (b,d,f,h,j,l), before and after seeding with MSCs cultured with FBS. (a,b,g,h) Pre-seeding images of both non-pored (a,b) and pored (g,h) scaffolds reveal no presence of MSCs. (c,d,i,j) After 7 days post-seeding, MSCs have adhered superficially to the non-pored scaffold (c,d) while in the pored one (i,j) a repopulation of the channels was already observed. (e,f,j,k) After 14 days post-seeding, MSCs were even more present on the surface of the non-pored scaffold (e,f), while they were able to penetrate deeper and with a great extent in the pored one (k,l). All the figures in the panel are the same scale; bar—100 µm.