Fig. 5. The PIR helix of the SLFN12 CTD is required for DNMDP-induced cancer cell killing.
a Schematic diagram of SLFN12 deletion mutants. b Seventy-two-hour DNMDP-response viability assay of SLFN12 truncation mutants deleting the PIR region expressed in HeLa-Res cells lacking endogenous SLFN12 expression. Data are plotted as mean values with error bars indicating +/−standard deviation of four replicates. c Anti-flag immunoblot analysis showing the expression levels of flag-tagged SLFN12 proteins, marked with arrows.