Fig. 3. Effect of H₂O₂ on the activation of MAPK signaling pathways in NS20Y cells.

a, c, and e Representative blots showing increased phosphorylation of JNK, p38, and ERK1/2 at 30 and 60 min following H₂O₂ (20 μM) treatment in NS20Y cells. b, d, and f Quantification analysis of the phosphorylation of JNK, p38, and ERK1/2 following H₂O₂ (20 μM) treatment in NS20Y cells (n = 4, P = 0.004, P < 0.001 and P = 0.016 versus the control, one-way ANOVA followed by Bonferroni’s post hoc test).