Fig. 4. Effect of MAPK signaling pathway blockade on the H₂O₂-mediated increase in ASIC1a expression.

a Representative blots and (b) quantification of ASIC1a protein expression in the presence or absence of H₂O₂ (20 μM) for 24 h in NS20Y cells and in the presence or absence of the MEK/ERK pathway inhibitor U0126 (10 μM) (n = 9,  P < 0.001 versus the group not treated with H₂O₂ or U0126 treatment, one-way ANOVA followed by Bonferroni’s post hoc test). U0126 or vehicle (0.1% DMSO) was added 1 h prior to and during H₂O₂ treatment. c Representative blots and d quantification of ASIC1a protein expression in NS20Y cells in the presence or absence of H₂O₂ (20 μM) for 24 h and in the presence or absence of the p38 MAPK inhibitor SB203580 (10 μM) (n = 8, P = 0.014 versus the group not treated with H₂O₂ or SB203580 treatment). SB203580 or vehicle (0.1% DMSO) was added 1 h prior to and during H₂O₂ treatment. e Representative blots and (f) quantification of ASIC1a protein expression in NS20Y cells in the presence or absence of H₂O₂ (20 μM) for 24 h and in the presence or absence of the JNK inhibitor SP600125 (10 μM) (n = 9, P < 0.001 versus the group not treated with H₂O₂ or SP600125 treatment, P < 0.001 versus H₂O₂ alone, one-way ANOVA followed by Bonferroni’s post hoc test). SP600125 or vehicle (0.1% DMSO) was added 1 h prior to and during H₂O₂ treatment.