Table 2.
pKD values and relative accessible US28 of A647-labeled CX3CL1 to different NLuc-US28-nanobody fusion constructs.
| US28 construct | pKD A647-CX3CL1 (Mean + SD) | Relative accessible US28 A647-CX3CL1 (Mean + SD) | pKD A647-CCL5 (Mean + SD) | Relative accessible US28 A647-CCL5 (Mean + SD) |
|---|---|---|---|---|
| NLuc-US28 WT | 9.6 ± 0.1 | 1 ± 0.0 | 8.8 ± 0.06 | 1 ± 0.0 |
| NLuc-US28-Irr Nb | 9.6 ± 0.1 | 0.96 ± 0.10 | 8.8 ± 0.1 | 1.02 ± 0.10 |
| NLuc-US28-VUN103 | 9.6 ± 0.06 | 1.30 ± 0.07 | 8.8 ± 0.1 | 1.45 ± 0.10 |
| NLuc-US28-Nb7 | 10.4 ± 0.02 | 1.37 ± 0.03 | 9.8 ± 0.1 | 1.61 ± 0.04 |