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. 2021 Jul 16;4:884. doi: 10.1038/s42003-021-02391-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a FBXO32 interacts with ATF2 in HEK293 cells. Immunoprecipitation performed with FBXO32 antibody or control IgG antibody from HEK293 cells transfected with the indicated plasmids followed by immunoblotting using the indicated antibodies. The results are from two independent experiments. b Immunoprecipitation from a human heart showing interaction of ATF2 with FBXO32. IgG-Mouse antibody was used as control. c Representative immunoblots showing that FBXO32 regulates ATF2 ubiquitination in HEK293 cells. FLAG-tagged ATF2 was co-transfected with HA-tagged ubiquitin (HA-Ub) and WT-FBXO32 or mutant-FBXO32 in HEK293 cells. Cell lysates were immunoprecipitated with anti-ATF2 antibody and immunoblotted with the indicated antibody. Data are representative of 3 independent transfections for WT-FBXO32 and mutant-FBXO32 (n = 2–3 replicates).