Figure 2.

Colons from Smad4^ΔLrig1 mice show no evidence of gross mucosal damage. A: representative hematoxylin and eosin (H&E) stains from Smad4^ΔLrig1 and control mice showing epithelial integrity. B: fluorescence in situ hybridization (FISH) staining with the pan-bacterial probe, Eub338 (red). Nuclei in light blue. Bacterial species indicated by solid arrowhead. Autofluorescent red blood cells indicated by open arrowhead. Border between lumen and epithelium demarcated with dashed line. C: Alcian blue (pH 2.5) stain for mucins (blue).