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. 2021 Mar 24;320(6):G936–G957. doi: 10.1152/ajpgi.00053.2021

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Published by the American Physiological Society

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Figure 4. — Loss of intestinal Smad4 expression is associated with altered levels, but not localization, of several Claudin proteins. A: Western blots (WB) from protein lysates generated from colon epithelium of five Smad4^ΔLrig1 and five control mice. B: quantification of relative protein density by WB. Protein density was compared between mouse genotypes by Mann–Whitney test. C: immunohistochemistry for SMAD4 protein (brown) in SMAD4+ control and Smad4^ΔLrig1 mice. D: immunofluorescence staining for indicated Claudin proteins. Claudin proteins in red, nuclei in green. Statistical significance is designated as nsP ≥ 0.05, *P < 0.05, **P < 0.01.

Figure 4. — Loss of intestinal Smad4 expression is associated with altered levels, but not localization, of several Claudin proteins. A: Western blots (WB) from protein lysates generated from colon epithelium of five Smad4^ΔLrig1 and five control mice. B: quantification of relative protein density by WB. Protein density was compared between mouse genotypes by Mann–Whitney test. C: immunohistochemistry for SMAD4 protein (brown) in SMAD4+ control and Smad4^ΔLrig1 mice. D: immunofluorescence staining for indicated Claudin proteins. Claudin proteins in red, nuclei in green. Statistical significance is designated as nsP ≥ 0.05, *P < 0.05, **P < 0.01.