Table 1.
Antibodies, qPCR primers, and fluorescence in situ hybridization probes utilized
| Antibodies | ||||
|---|---|---|---|---|
| Target Protein | Company | Product Number | Dilution | References |
| SMAD4 | Abcam | 40759 | 1:1,000 (IHC [FFPE/Cryo])1:500 (WB) | (14) |
| Claudin 2 | Invitrogen | 51-6100 | 1:125 (IF [Cryo])1:1,000 (WB) | (41) |
| Claudin 3 | Invitrogen | 34-1700 | 1:150 (IF [FFPE])1:500 (WB) | (45) |
| Claudin 7 | Invitrogen | 34-9100 | 1:250 (IF [Cryo])1:20,000 (WB) | (45) |
| Claudin 8 | Abcam | 183738 | 1:1000 (IF [FFPE])1:1000 (WB) | (42, 43, 46) |
| FITC | Abcam | ab6656 | 1:1000 (IF [Cryo]) | (44) |
|
qPCR Primers | ||
|---|---|---|
| Target Gene | Forward (5′ to 3′) | Reverse (5′ to 3′) |
| Cldn2 | tgaacacggaccactgaaag | ttagcaggaagctgggtcag |
| Cldn3 | gtggccactgcagctactt | gtttcatggtttgcctgtctc |
| Cldn4 | ttttgtggtcaccgactttg | tgtagtcccatagacgccatc |
| Cldn7 | tgtcttgtggagggcttga | caagcatggccattgaaa |
| Cldn8 | gggcctggggataaaagag | aatccttaagctgtttttaggcaat |
| Smad7 | acccccatcaccttagtcg | gaaaatccattgggtatctgga |
| Pmm1 (Reference Gene) | gggtggctctgactactctaagat | acacgtagtcaaacttctcaatgact |
| Fluorescence In Situ Hybridization (FISH) Probes | |
|---|---|
| Eub338 | 5′-Cy3-GCTGCCTCCCGTAGGAGT-3′ |
| Non-Eub (Negative Control) | 5′-Cy3-CGACGGAGGGCATCCTCA-3′ |
Cryo, cryopreserved (fresh frozen) tissue; FFPE, formalin-fixed and paraffin-embedded; IF, immunofluorescence; IHC, immunohistochemistry; WB, Western blot.