Figure 4.

Endogenous neuregulin-4 limits proinflammatory cytokine production in M1 macrophages. Inflammatory gene expression was analyzed by qPCR in BM-Mɸs generated from NRG4-KO mice and wild-type littermates at 6 and 24 h poststimulation. Data are presented relative to wild-type littermates at each respective time point normalized to 1 (indicated by the dashed red line). A: at 6 h post-M1 activation, no cytokines are significantly altered in NRG4-KO BM-Mɸs. At 24 h post-M1 activation, Tnf expression is significantly elevated. B: NRG4 knockout was confirmed in BM-Mɸs by qPCR. n = cultures from 4 or 6 mice/genotype, from four separate litters; *P < 0.05; ****P < 0.0001.