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. 2021 Apr 26;320(6):E1107–E1118. doi: 10.1152/ajpendo.00281.2020

Figure 3.

Figure 3.

Relative transcript expression of proteins related to the insulin signaling cascade, glucose uptake, and steroid hormone receptors in white adipose tissue, liver, and skeletal muscle of placebo and testosterone-treated female pigs. Relative transcript expression in subcutaneous white adipose tissue (scWAT; A), visceral WAT (vWAT; B), liver (C), and skeletal muscle (D) in placebo (black bars) and testosterone-treated (gray bars). qPCR was conducted on tissues collected at D13 of treatment. A two-way ANOVA was used for statistical analysis. We used n = 5 for testosterone-treated females and n = 6 for placebo females for all parameters assessed in this figure. *P ≤ 0.05. AKT2: protein kinase B isoform 2; AR: androgen receptor; ESR1: estrogen receptor α; FOXO1: forkhead box ortholog 1; GLUT4: glucose transporter 4; INSR: insulin receptor; IRS1: insulin receptor substrate 1; PDK1: phosphoinositide-dependent kinase 1; PI3K: phosphoinositide 3 kinase; PPARG: peroxisome proliferator-activated receptor gamma; test-treated: testosterone-treated.