Figure 6.

Relative protein abundance of protein kinase B (AKT) and forkhead box ortholog 1 (FOXO1) in skeletal muscle of placebo and testosterone-treated (Test-treated) females. Phosphorylated(Ser473) AKT (pAKT^Ser473) and total AKT (A); phosphorylated(Ser256) FOXO1 (pFOXO1^Ser256) and total FOXO1 (B). An unpaired t test was utilized for statistical analysis between treatment groups for each protein of interest. For AKT and FOXO1, n = 5 for testosterone-treated females and n = 6 for placebo females. Western blotting was conducted on tissues collected at D13 of treatment. #0.05 < P ≤ 0.10. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.