Figure 3.
Contraction-stimulated increases in the phosphorylation of AMPK and p38 are similar in muscle-specific knockout of sirtuin 1 (mKO) and wild-type (WT) mice. A–D: representative images and quantification of pAMPKT172 (A and C) and p-p38T180/Y182 (B and D) from the rested (Rest) and contracted (Contract.) paired extensor digitorum longus (EDL) of female (A and B) and male (C and D) WT and mKO mice; analysis was conducted in the same muscles used for 2-deoxyglucose uptake analysis. Protein phosphorylation was corrected for total abundance, and the data are presented as relative (rel.) to WT-Rest. Dotted line indicates spliced images from gels that have been exposed together for the same duration of time. Statistics: data were analyzed by a repeated measures two-way ANOVA. *P < 0.05, main effect of contraction. Male: WT-AMPK, n = 5; WT-p38, n = 6; mKO-AMPK, n = 6; mKO-p38, n = 6. Female: WT-AMPK, n = 5; WT-p38, n = 6; mKO-AMPK, n = 5; mKO-p38, n = 5. Data are presented as means ± SE.