Figure 6.

Acute pan-inhibition of deacetylases in L6 myotubes does not impair AMPK-stimulated GLUT4 translocation to the plasma membrane. Plasma membrane (PM) GLUT4 abundance in basal and MK8722 (10 µM)-stimulated L6 myotubes treated without and with NAM at 10 mM and 20 mM is shown. There was a main effect of MK8722 but no effect of nicotinamide (NAM) treatment in L6 myotubes. Data are presented as relative to basal-control. Statistics: data were analyzed by a repeated measures two-way ANOVA. *P < 0.05, main effect of MK8722 stimulation. Data represent n = 3 in all groups. Data are presented as means ± SE.