Figure 4.

HBK mice display lower systolic blood pressure. Telemetry data for flox and HBK mice were plotted as 1-h averages of 3 consecutive days. Inactive and active period BP and HR for eACh mouse were calculated by averaging values during the inactive (ZT0-12) or active (ZT12-0) 12-h periods and are indicated by white and gray shading, respectively. Systolic blood pressure (SBP; A), diastolic blood pressure (DBP; B), mean arterial pressure (MAP; C), and heart rate (HR; D) in flox controls and HBK mice are shown as traces (left) with 12-h inactive and active averages (right). Two-way repeated-measures ANOVA was used to compare time of day and genotype effects with Sidak’s post hoc test for multiple comparisons (n = 4, *P < 0.05). Significant two-way ANOVA results are included in graphs. Time of day had a significant effect on (A) SBP, (B) DBP, (C) MAP, and (D) HR in both flox and HBK mice. Genotype had a significant effect on SBP (A) with the post hoc test showing significance between genotypes during the inactive period. *P < 0.05 flox vs. HBK. HBK, hepatocyte-specific Bmal1 knockout.