Figure 2.

B₁₂ deficiency compromises trabecular and cortical architecture in load bearing bones and a single injection of B₁₂ in the mother rescues compromised bone architecture in the offspring. Schematic representation of the experimental protocol used (A), histological and histomorphometric analysis of the tibia (B), serum osteocalcin levels (C) and serum dpd levels (D) in 8-wk-old offspring born from mothers that received a single injection of vehicle or B₁₂ at embryonic day 12.5 are sown. Micro-CT analysis of the proximal tibia: bone volume/total volume (BV/TV)% (E), trabecular thickness (Tb.Th.) (F), trabecular number (Tb.N.) (G), trabecular patterning factor (Tb.Pf.) (H) is shown. I: representative micro-CT images of midshaft in tibia of 8-wk-old offspring born from mothers that received a single injection of vehicle or B₁₂ at embryonic day 12.5. Cortical thickness (Ct.Th.) (J), endo-osteal perimeter (End. Pm.) (K), medullary area (Med. Ar.) (L), and moment of inertia (MMI; M) are shown. Means ± SE is shown. n = 8 (B–D), n = 10 (E–M) female mice each group. *P < 0.05. #P < 0.01.