Figure 4.

Effect of B₁₂ deficiency and supplementation on the skeletal muscle. Gastrocnemius weight (A), tibialis anterior weight (B), extensor digitorum longus weight (C), soleus muscle weight (D), soleus total fiber numbers (E), grip strength (F), representative image of soleus muscle fiber histology with ATPase staining showing distribution of soleus muscle fibers (G), soleus Type I (H), and soleus Type IIa fibers cross-sectional area (CSA; I) in 8-wk-old offspring born from mothers that received a single injection of vehicle or B₁₂ at embryonic day 12.5 are shown. Means ± SE is shown. n = 3–10 mice each group. *P < 0.05.