Figure 2.

Triiodothyronine (T3) pretreatment attenuates hyperoxia-induced lung injury, inflammation, and oxidants in wild-type (WT) mice compared with mice treated with propylthiouracil (PTU). WT mice were nebulized with triiodothyronine (T3, 40 µg/kg) for 1 day or 3 days or propylthiouracil (PTU, 100 µg/kg) for 3 days before 72 h continuous hyperoxia exposure. Control mice were exposed to room air (RA). A: total cells recovered from bronchoalveolar lavage (BAL) were counted. B: lung permeability was assessed by BAL protein content. C) lactate dehydrogenase (LDH) activity assays were performed on BAL fluid. D: oxidant generation was detected by Amplex Red from BAL fluid. IL-1β (E) and IL-6 (F) were detected by ELISA in BAL fluid. The values are expressed as means ±SD and analyzed by Mann–Whitney test (n = 6 for each group). *P < 0.05, vs. RA; **P < 0.05, vs. hyperoxia without pretreatment.