Figure 3.

Triiodothyronine (T3) pretreatment increases mitochondrial biogenesis/fusion/mitophagy and decreases ER stress and apoptosis compared to mice treated with propylthiouracil (PTU). WT, PINK1^−/−, and Parkin^−/− mice were nebulized with triiodothyronine (T3, 40 µg/kg) for 3 days or no pretreatment before 72 h continuous hyperoxia exposure. Lysates were isolated and immunoblotted against antibodies as listed. β-Actin was used as protein loading control. Quantification based on densitometry of the Western blot is shown in Supplemental Fig. S1. The uncut Western blots listed in the Supplemental Fig. S3. PINK1, PTEN-induced kinase I; RA, room air; WT, wild-type.