Figure 5.

Triiodothyronine (T3) pretreatment increases in mitochondrial anti-ROS potential, biogenesis and mitophagy under hyperoxia are dependent upon PINK1. WT and PINK1^−/− mice were nebulized with triiodothyronine (T3, 40 µg/kg) for 3 days before 72 h continuous hyperoxia exposure. Lysates were isolated and immunoblotted against antibodies as listed. β-Actin was used as protein loading control. Quantification based on densitometry of the western blots shown in Supplemental Fig. S2. The uncut Western blots are shown in Supplemental Fig. S4. PINK1, PTEN-induced kinase I; ROS, reactive oxygen species; WT, wild-type.