Figure 6.

Triiodothyronine (T3) agonist GC-1 pretreatment prevents hyperoxia induced lung injury and aids recovery. WT mice were orally gavaged with GC-1 (5 mg/kg) and then exposed to RA or to hyperoxia for 72 h (H3), followed by a recovery phase (R1–3, 1, 2, 3, and R6, 6 days posthyperoxia). Cells recovered from bronchoalveolar lavage (BAL) were counted as BAL total cell counts (A). B: lung permeability was assessed by BAL protein content. C: lactate dehydrogenase (LDH) activity assays were performed on BAL fluid. D: oxidant generation was detected by Amplex Red from BAL fluid. IL-6 (E) and IL-1β (F) was detected by ELISA in BALF. G: structural formula of GC-1. The values are expressed as means ± SD and analyzed by Mann–Whitney test (n = 4 for each group). *P < 0.05 vs. no GC-1 RA; **P < 0.05 vs. no GC-1 H3; #P < 0.05 vs. corresponding no GC-1. RA, room air.