Figure 7.

GC-1 pretreatment prevents hyperoxia induced lung injury and aids recovery. Wild-type (WT) mice were orally gavaged with GC-1 (5 mg/kg) and then exposed to RA or to hyperoxia for 72 h (H3), followed by a recovery phase (R1-3, 1, 2, 3, and R6, 6 days posthyperoxia). Lysates from mouse lungs were immunoblotted against the listed Abs. The uncut Western blots are shown in Supplemental Fig. S5. All of the fold changes are compared with the room air control set as 1. For LC3B, comparison was made between the two bands of LC3B, but not to the loading control; the ratio of the two bands indicates the change in autophagy.