CFR negatively correlates with seven cardiovascular disease-related proteins (A) that are lower in MVs from patients with high CFR (B). Olink proteomic CVD II and III panels were used to measure 180 cardiovascular biomarkers. A: correlation plots showing normalized protein expression (NPX) values of the significantly correlated proteins (x-axis), with corresponding CFR (y-axis), P < 0.05. AXL: n = 143, BAFF: n = 122, CD163: n = 137, IGFBP7: n = 166, NEMO: n = 130, perlecan: n = 145, resistin: n = 157. B: patients from the high CFR group have significantly lower biomarker concentration (NPX values) in plasma MVs than the low CFR group. Scatter plots presenting median ± 95% CI. Mann–Whitney U test for unpaired data. *Statistically significant, P < 0.05. AXL: n = 62 (low CFR), 81 (high CFR); BAFF: n = 52 (low CFR), 70 (high CFR); CD163: n = 58 (low CFR), 79 (high CFR); IGFBP7: n = 75 (low CFR), 91 (high CFR); NEMO: n = 62 (low CFR), 68 (high CFR); perlecan: n = 59 (low CFR), 86 (high CFR); resistin: n = 68 (low CFR), 89 (high CFR). BAFF, B cell activating factor; CFR, coronary flow reserve; IGFBP7, insulin-like growth factor-binding protein 7; MVs, microvesicles; NEMO, NF-κB essential modulator.