Figure 3.

Increasing miR-195 reduces the numbers of Tuft and Paneth cells in intestinal organoids. A: immunostaining of Tuft (DCLK1-positive) and Paneth (lysozyme-positive) cells in intestinal organoids: littermates (a); miR195-Tg mice (b); and quantitative data of Tuft and Paneth cells (c). Primary enterocytes were isolated from the proximal small intestine, and changes in Tuft and Paneth cells were examined by immunostaining assays on day 5 after primary cultures. Values are the means ± SE (n = 5 replicates). *P < 0.05 compared with littermate mice. Scale bar: 25 μm. B: Goblet cells (Mucin 2-positive cells) and enterocyte differentiation (marked by villin immunostaining) in intestinal organoids described in A. Scale bar: 25 μm. Experiments were repeated in five littermate or miR195-Tg mice and showed similar results. Statistical significance was analyzed using unpaired, two-tailed Student’s t test. DCLK1, double cortin-like kinase 1; miR195-Tg, miR-195 transgenic.