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. 2021 Mar 31;320(6):C1042–C1054. doi: 10.1152/ajpcell.00597.2020

Table 1.

Primers used for DCLK1 luciferase reporters and miR-195 construct

Primers Sequences
Luciferase assay
hDCLK1 3′UTR-WT
 Forward 5′-GCGCCTCGAGGGTACAAGGCGCAGCCAG-3′
 Reverse 5′-GCGCGTCGACGTGTTATCACTTCCGGATGC-3′
hDCLK1 3′UTR-F1
 Forward 5′-GCGCCTCGAGGGTACAAGGCGCAGCCAG-3′
 Reverse 5′-GCGCGTCGACTTCCAGCCCTGCAGAGCACC-3′
hDCLK1 3′UTR-F2
 Forward 5′-GCGCCTCGAGGGAATTTCAAACAGAAAGAGGG-3′
 Reverse 5′-GCGCGTCGACGTGTTATCACTTCCGGATGC-3′
Cloning
 miR195 Forward 5′-CGACGTTTAAACGCGGATCCAGATCTGAA-3′
 miR195 Reverse 5′-CTGGGTAAAAGCTAGCGAATTCCGTTCC-3′
Genotyping
 Forward 5′-AGAAACTGGGCTTGTCGAGACAGA-3′
 Reverse 5′-AAGACTCTACTTTGCTCTGTGGGG-3′

DCLK1, double cortin-like kinase 1; UTR, untranslated region; WT, wild type.