Table 1.
Primers | Sequences |
---|---|
Luciferase assay | |
hDCLK1 3′UTR-WT | |
Forward | 5′-GCGCCTCGAGGGTACAAGGCGCAGCCAG-3′ |
Reverse | 5′-GCGCGTCGACGTGTTATCACTTCCGGATGC-3′ |
hDCLK1 3′UTR-F1 | |
Forward | 5′-GCGCCTCGAGGGTACAAGGCGCAGCCAG-3′ |
Reverse | 5′-GCGCGTCGACTTCCAGCCCTGCAGAGCACC-3′ |
hDCLK1 3′UTR-F2 | |
Forward | 5′-GCGCCTCGAGGGAATTTCAAACAGAAAGAGGG-3′ |
Reverse | 5′-GCGCGTCGACGTGTTATCACTTCCGGATGC-3′ |
Cloning | |
miR195 Forward | 5′-CGACGTTTAAACGCGGATCCAGATCTGAA-3′ |
miR195 Reverse | 5′-CTGGGTAAAAGCTAGCGAATTCCGTTCC-3′ |
Genotyping | |
Forward | 5′-AGAAACTGGGCTTGTCGAGACAGA-3′ |
Reverse | 5′-AAGACTCTACTTTGCTCTGTGGGG-3′ |
DCLK1, double cortin-like kinase 1; UTR, untranslated region; WT, wild type.