Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 19;320(6):F1133–F1151. doi: 10.1152/ajprenal.00614.2020

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 the American Physiological Society

PMC Copyright notice

Figure 2. — Sacubitril/valsartan (Sac/Val) treatment improved glomerulosclerosis in db/db mice. A: representative images of periodic acid-Schiff staining of kidney sections. The mesangial expansion score was defined by the percentage of the mesangial area in the glomerular tuft area. The mesangial area was determined by assessment of periodic acid-Schiff-positive and nucleus-free areas in the mesangium; n = 4 per group. Scale bar = 100 µm. B and C: immunofluorescence staining of kidney sections for fibronectin (B) and collagen type IV (C). Data were quantified using the ratio of total pixel intensity vs. glomerular area; n = 6 per group. D: quantitative PCR analysis of mRNA levels of fibronectin and collagen type IV in the kidneys from each group; n = 6−9 per group. E: quantitative PCR analysis of mRNA levels of genes involved in profibrotic factors and signaling pathways, transforming growth factor-β (TGF-β), connective tissue growth factor (CTGF), plasminogen activator inhibitor-1 (PAI1), and α-smooth muscle actin (α-SMA); n = 6−9 per group. Data are presented as means ± SE. Veh, vehicle.

Figure 2. — Sacubitril/valsartan (Sac/Val) treatment improved glomerulosclerosis in db/db mice. A: representative images of periodic acid-Schiff staining of kidney sections. The mesangial expansion score was defined by the percentage of the mesangial area in the glomerular tuft area. The mesangial area was determined by assessment of periodic acid-Schiff-positive and nucleus-free areas in the mesangium; n = 4 per group. Scale bar = 100 µm. B and C: immunofluorescence staining of kidney sections for fibronectin (B) and collagen type IV (C). Data were quantified using the ratio of total pixel intensity vs. glomerular area; n = 6 per group. D: quantitative PCR analysis of mRNA levels of fibronectin and collagen type IV in the kidneys from each group; n = 6−9 per group. E: quantitative PCR analysis of mRNA levels of genes involved in profibrotic factors and signaling pathways, transforming growth factor-β (TGF-β), connective tissue growth factor (CTGF), plasminogen activator inhibitor-1 (PAI1), and α-smooth muscle actin (α-SMA); n = 6−9 per group. Data are presented as means ± SE. Veh, vehicle.