Figure 7.

High sodium (HS)-induced decrease in epithelial Na⁺ channel (ENaC) activity in the distal convoluted tubule (DCT) is larger in Kir5.1 knockout (KO) mice than in wild-type (WT) mice. A: set of gap-free recordings showing amiloride-sensitive Na⁺ currents in the late DCT (DCT2)/connecting tubule (CNT) measured at −60 mV with the perforated whole cell patch in WT and Kir5.1 KO mice (male only) on low sodium (LS; 7 days), normal sodium (NS), and high sodium (HS; 7 days), respectively. B: scatterplot summarizing the results of experiments in which amiloride-sensitive (10 µM) Na⁺ currents were measured at −60 mV with perforated whole cell recordings in the DCT2/CNT of WT and Kir5.1 KO mice on NS, LS (7 days), or HS diets for 7 or 14 days, respectively. Mean values and SEs are shown on the left of each column. *P < 0.05 and **P < 0.01 as determined by two-way ANOVA.