Table 1.
Caudate-putamen medium spiny neuron electrophysiological properties from females and males exposed to vehicle or estradiol during the perinatal period
| Property | Vehicle | Estradiol | Statistics (F, P) |
|---|---|---|---|
| Resting membrane potential, mV | F: −82.8 ± 1.7 (24) | −86.1 ± 1.6 (29) | 0.11, 0.74 |
| M: −84.2 ± 1.3 (28) | −86.6 ± 1.4 (37) | 0.38, 0.53 | |
| 3.48, 0.06 | |||
| Input resistance, MΩ | F: 260.2 ± 26.9 (24) | 213.9 ± 14.3 (29) | 0.90, 0.34 |
| M: 245.2 ± 15.3 (28) | 265.8 ± 17.2 (37) | 2.98, 0.09 | |
| 0.43, 0.51 | |||
| Rectified range input resistance, MΩ | F: 200.1 ± 13.7 (24) | 187.1 ± 11.1 (29) | 0.72, 0.39 |
| M: 209.6 ± 12.2 (28) | 218.0 ± 12.1 (37) | 2.61, 0.11 | |
| 0.03, 0.85 | |||
| Inward rectification, MΩ | F: 62.7 ± 14.8 (24) | 26.8 ± 4.6 (29) | 7.60, 0.0068 |
| M: 35.5 ± 4.7 (28) | 47.8 ± 8.5 (37) | 0.12, 0.73 | |
| 1.82, 0.18 | |||
| % Inward rectification, % | F: 82.2 ± 2.8 (24) | 89.0 ± 1.4 (29) | 6.70, 0.0109 |
| M: 86.5 ± 1.5 (28) | 83.6 ± 1.7 (37) | 0.09, 0.76 | |
| 1.08, 0.18 | |||
| Time constant of the membrane, ms | F: 16.6 ± 2.5 (24) | 12.0 ± 1.1 (29) | 6.70, 0.07 |
| M: 13.2 ± 1.0 (28) | 14.1 ± 1.2 (37) | 0.09, 0.68 | |
| 1.08, 0.21 | |||
| Capacitance, pF | F: 61.6 ± 4.8 (24) | 57.6 ± 4.6 (29) | 0.21, 0.65 |
| M: 54.9 ± 3.1 (28) | 54.4 ± 3.0 (37) | 1.63, 0.20 | |
| 0.33, 0.56 | |||
| Rheobase, pA | F: 101.9 ± 12.8 (24) | 161.5 ± 16.7 (29) | 5.5, 0.0204 |
| M: 111.7 ± 10.5 (28) | 112.2 ± 9.3 (37) | 2.5, 0.12 | |
| 5.7, 0.0182 | |||
| Delay to first AP, ms | F: 310.1 ± 27.0 (24) | 356.8 ± 22.8 (29) | 1.08, 0.30 |
| M: 344.1 ± 17.0 (28) | 348.9 ± 14.8 (37) | 0.42, 0.52 | |
| 1.64, 0.20 | |||
| AP threshold, mV | F: −48.4 ± 1.7 (24) | −44.4 ± 1.9 (29) | 3.64, 0.0589 |
| M: −45.8 ± 2.0 (28) | −48.7 ± 1.6 (37) | 0.24, 0.63 | |
| 0.09, 0.76 | |||
| AP width at half-peak amplitude, ms | F: 2.6 ± 0.2 (24) | 2.5 ± 0.1 (29) | 1.10, 0.30 |
| M: 3.0 ± 0.2 (28) | 2.5 ± 0.1 (37) | 3.41, 0.0672 | |
| 3.09, 0.0813 | |||
| AHP peak amplitude, mV | F: −7.6 ± 0.5 (24) | −8.6 ± 0.3 (29) | 6.5, 0.0120 |
| M: −8.4 ± 0.4 (28) | −7.5 ± 0.3 (37) | 0.2, 0.65 | |
| 0.1, 0.73 | |||
| AHP time to peak, ms | F: 33.5 ± 3.4 (24) | 33.7 ± 2.1 (29) | 1.1, 0.29 |
| M: 37.5 ± 2.3 (28) | 32.7 ± 1.7 (37) | 0.4, 0.52 | |
| 1.0, 0.31 | |||
| FI slope, Hz/nA | F: 207.1 ± 24.2 (24) | 167.2 ± 7.0 (29) | 9.4, 0.0027 |
| M: 157.9 ± 8.5 (28) | 212.3 ± 16.6 (37) | 0.0, 0.88 | |
| 0.2, 0.65 | |||
| mEPSC frequency, Hz | F: 2.3 ± 0.3 (21) | 3.3 ± 0.5 (24) | 0.32, 0.57 |
| M: 2.0 ± 0.3 (24) | 3.6 ± 0.8 (7) | 0.00, 0.99 | |
| 5.9, 0.0178 | |||
| mEPSC amplitude, pA | F: 10.0 ± 0.3 (21) | 10.8 ± 0.5 (24) | 0.31, 0.58 |
| M: 10.0 ± 0.7 (24) | 11.5 ± 1.2 (7) | 0.31, 0.58 | |
| 2.6, 0.11 | |||
| mEPSC decay, ms | F: 2.7 ± 0.2 (21) | 2.7 ± 0.2 (24) | 0.86, 0.36 |
| M:3.1 ± 0.2 (24) | 2.7 ± 0.4 (7) | 0.42, 0.52 | |
| 0.7, 0.40 |
Electrophysiological properties recorded from caudate-putamen MSNs from prepubertal female and male rats exposed to either vehicle or estradiol in the perinatal period. Values are means ± SE. Numbers in parentheses indicate the number of neurons in each group (experimental n). Statistics column lists F and P values for interaction, sex, and hormone from top to bottom for a two-way ANOVA analysis with sex and estradiol exposure as factors. Bold font indicates statistical significance. AHP, afterhyperpolarization; AP, action potential; F, female; FI, evoked firing rate-to-positive current curve; M, male; mEPSC, miniature excitatory postsynaptic current.