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. 2021 Jul 17;14:368. doi: 10.1186/s13071-021-04809-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — MLSA of B. miyamotoi Hetao-1 and other relapsing fever borreliae. A phylogenetic inference of the concatenated housekeeping gene sequences of the representative relapsing fever borreliae is shown. The arrow indicates the B. miyamotoi strain Hetao-1 isolated in this study. Consensus sequences for the eight housekeeping genes were isolated from the draft genome sequence of B. miyamotoi strains Hetao-1, trimmed to lengths and concatenated in the order: clpA, clpX, nifS, pepX, pyrG, recG, rplB, and uvrA according to the Borrelia PubMLST database. For phylogenetic reconstruction, the maximum likelihood model based on the Kimura two-parameter model with MEGA 6.0 was used with 1000 bootstrap replicates. Borrelia turcica IST7 was used as outgroup. The ST number designated in each strain indicates the “sequence type” registered in the Borrelia PubMLST database