Table 2.

Overview of sequencing and quality control of samples

Sample	Raw reads	Clean reads	Mapped reads	Q30 (%)	OD260/280 ratio	Size (bp)	Total amount (ng)b
P1	71,752,862	70,830,830	50,966,866	90.56	1.84a	317	38.6
P2	81,810,500	80,354,146	59,939,550	90.48	1.92a	307	41.9
P3	81,942,214	80,005,894	55,444,504	90.66	1.82a	300	33.6
C1	80,311,782	79,697,224	61,590,054	91.77	1.85a	309	29.4
C2	90,455,988	89,212,696	68,539,836	91.95	1.88a	299	36.6
C3	81,985,938	81,104,462	62,490,876	91.86	1.81a	302	42.3

P1-3 represented samples of PMOF patients; C1-3 represented samples of healthy participates

^aThe O.D. A260/A280 ratio over 1.8 was acceptable for pure RNA

^bThe libraries were adjusted to 10 nM before cluster generation