Fig. 4.

Allogeneic HIP iECs alleviate heart failure in cryoinfarcted hearts. (A and B) BALB/c mice underwent cryoinfarction of their anterolateral left ventricle (A) and some subsequently received injections of allo iECs or alloHIP iECs (B) into the border zone. (C) Over a study period of 28 d, graft survival was monitored and iEC integration into host myocardium was assessed. (D and E) The survival of FLuc⁺ allo iEC grafts (D, 11 animals) and alloHIP iEC grafts (E, 10 animals) was longitudinally followed by BLI. BLI signals of individual animals are plotted, representative pictures are shown. (F–I) Invasive PV loop analyses (mean ± SD, 10 baseline animals, 4 infarct animals, 10 allo iEC animals, 10 alloHIP animals; ANOVA with Bonferroni post hoc test). Parameters included EF (F), SV (G), SW (H), and CO (I). (J) Immunofluorescence staining of the infarct border zone did not detect any FLuc⁺ graft cells in recipients of allo iECs. However, all animals that received alloHIP iECs showed engraftment localized to the endothelial cell layers of larger and smaller intramyocardial vessels (representative pictures of 11 animals in the allo and 10 animals in the alloHIP group).