Fig. 5.

Immature NK cells preferentially differentiate into ieILC1-like cells. (A–C) CD56^bright or CD56^dim NK cells were sorted from peripheral blood of healthy donors and cocultured with PCI-13 with or without IL-15 (10 ng/mL), as described in Fig. 4B. (A) (Left) Dot plots show a representative example of the expression of CD49a and CD103 on NK cells after the 4-d coculture. Graphs show cumulative results of (Right) the percentage and (B) Mean fluorescence intensity (MFI) of CD49a, and (C) the percentage of CD49a⁺CD103⁺ cells, after the 4-d coculture, from three independent experiments, each using NK cells from a different donor. Graphs were expressed as mean ± SEM, and P values are shown. (D) Stage 4b (CD94⁺NKp80⁺CD16⁻) and two populations of stage 5 NK cells (CD94⁺NKp80⁺CD16⁺ and CD94⁻NKp80⁺CD16⁺) were sorted from peripheral blood of healthy donors and cocultured with PCI-13 and IL-15 (10 ng/mL), as described in Fig. 4B. Dot plots show a representative example, from three independent experiments, of the expression of CD49a and CD103 on NK cells after the 4-d coculture.