Table 5.
Rules for algorithmic score determination
| Feature | Points |
| REF outliers* | −1 |
| HET outliers* | −1 |
| VAR outliers* | −1 |
| HET results have big variance† | −1 |
| VAR results have big variance† | −1 |
| REF and B6 results are different‡ | −1 |
| REF and VAR results overlap§ | −1 to −3 |
| B6 and VAR results overlap§ | −0.5 |
| HET results more similar than REF results to B6 results¶ | −1 to −2 |
| VAR results more similar than REF results to B6 results# | −1 to −2 |
| Magnitude of change less than 2-fold for FACS B-1 B cell phenotype | −0.5 |
| Magnitude of change less than 1.5-fold for FACS B-1 B cell phenotype | −3 |
| Magnitude of change less than 2-fold for FACS B-2 B cell phenotype | −0.5 |
| Magnitude of change less than 1.5-fold for FACS B-2 B cell phenotype | −3 |
| Magnitude of change less than 2-fold for FACS DC phenotype | −0.5 |
| Insignificant position superpedigree result | −1 |
| Significant‖ position superpedigree result (only minority of pedigrees contributed) | −1 |
| In opposite direction of significant position superpedigree result | −3 |
| Significant position superpedigree result | 1.5 |
| In opposite direction of significant gene superpedigree result (null alleles) | −3 |
| Insignificant gene superpedigree result (null allele) | −1 |
| Significant gene superpedigree result (null alleles) | 1 |
| In opposite direction of significant gene superpedigree result (null+missense alleles) | −0.5 |
| Insignificant gene superpedigree result (null+missense alleles) | −0.5 |
| Significant gene superpedigree result (null+missense alleles) | 0.5 |
| In opposite direction of significant gene superpedigree result (missense alleles) | −0.5 |
| Insignificant gene superpedigree result (missense alleles) | −0.5 |
| Significant gene superpedigree result (missense alleles) | 0.5 |
| Significant selective gene superpedigree result with more than two pedigrees | 3 |
| Significant selective gene superpedigree result with two pedigrees | 2 |
| In opposite direction of insignificant selective gene superpedigree result | 1 |
| In opposite direction of significant selective gene superpedigree result with two pedigrees | −2 |
| In opposite direction of significant selective gene superpedigree result with more than two pedigrees | −3 |
| Insignificant selective gene superpedigree result | −1 |
| Significant selective gene superpedigree result exists for other phenotypes | 0.5 |
| Mutation is linked to a more damaging mutation | −1 |
| Mutation is linked to an excluded mutation | 1 |
| Mutation is linked to a verified mutation | −3 |
| No corresponding trimmed result | −3 |
| Phenotype is driven by another mutation | −1 |
B6, C57BL/6J.
A screen result is considered as an outlier if its value is outside the range of mean ± 3 × standard deviation (SD).
The variance of REF, HET, or VAR results is considered big if the SD is more than 30% of the absolute difference between the maximum screening result and the minimum screening result.
REF and B6 results are considered different if the absolute difference between the REF mean and B6 mean is more than 2 × REF SD and 2 × B6 SD.
REF or B6 results overlap with VAR results if they are within the range of VAR mean ± 1 × VAR SD.
HET results are considered more similar than REF results to B6 results if the absolute difference between the B6 mean and REF mean is more than half of REF SD and more than the absolute difference between HET mean and B6 mean.
VAR results are considered more similar than REF results to B6 results if the absolute difference between the B6 mean and REF mean is more than half of REF SD and more than the absolute difference between VAR mean and B6 mean.
Significant pedigree refers to linkage analysis of a pedigree or superpedigree by AMM in which P < 0.05 for a mutation-phenotype association.