Fig. 7.

Highly multiplexed in situ hybridization reveals subsets of molecularly and functionally diverse projection neurons in the superficial dorsal horn. (A–L) Representative superficial dorsal horn section illustrates Retrobead (RB)-labeled projection neurons from heat (Fos) and chloroquine (tdT)-stimulated TRAP2 mice. A subset of projection neurons is activated by heat (50 °C), but not chloroquine (A–F); other subsets are activated by chloroquine only (G), both heat and chloroquine (H–J), or neither stimulus (K and L). Each functionally defined subset includes projection neurons that express varying combinations of the genes Tacr1, Cck, Nptx2, and Crh.