Table 2. Expression of genes in stimulated adherent THP-1 cells compared to the unstimulated adherent cells at 72 h.

Statistical analysis was performed using unpaired T test with Welch’s correction. Fold change (2^−ΔΔCt) values higher than 1 indicate upregulation, whereas values between 0 and 1 indicate downregulation of mRNA transcripts in stimulated cells.

THP-1 cells adhered
Stimulation	mRNA	Roles	Fold change (2−ΔΔCt) Stimulated cells vs Control	P value
50 nM 1,25(OH)2 D3 (72 h)	CD14	Macrophage marker	650.9	0.0026
	CD36	Macrophage marker	3.13	0.0073
	HLA-DRA	Antigen presentation	0.09	0.0011
	PKD2	Mechanosensory non-selective cation channel	2.47	0.0096
	TCF3	Transcription factor (unknown roles in macrophages)	0.63	0.0134
	TCF4	Transcription factor (unknown roles in macrophages)	0.48	0.0032
	NFAT2	Transcription factor (undefined roles in macrophages)	0.52	0.0458
	TCF7L2	Transcription factor (proliferation and differentiation )	2.63	0.0313
	LEF-1	Transcription factor (proliferation and differentiation )	0.3	0.0491
Vibrations 1,000 Hz (30–60 nm) (72 h)	HLA-DRA	Antigen presentation	0.07	0.0022
	PIEZO1	Mechanosensory channel	11.44	0.0247
	PKD2	Mechanosensory non-selective cation channel	0.6	0.0236
	NFAT2	Transcription factor (undefined roles in macrophages)	0.12	0.0004
	TCF3	Transcription factor (unknown roles in macrophages)	4.73	0.04
50 nM 1,25(OH)2 D3 + Vibrations 1,000 Hz (30–60 nm) (72 h)	CD14	Macrophage marker	542.09	0.0011
	CD36	Macrophage marker	2.27	0.0227
	HLA-DRA	Antigen presentation	0.09	0.002
	PKD2	Mechanosensory non-selective cation channel	1.95	0.0013
	TCF3	Transcription factor (unknown roles in macrophages)	0.69	0.0232
	TCF4	Transcription factor (unknown roles in macrophages)	0.37	0.0016
	NFAT2	Transcription factor (undefined roles in macrophages)	0.31	0.0006
	TCF7L2	Transcription factor (proliferation and differentiation)	2.36	0.0116