Table 3. Comparison of THP-1 gene expression between different treatments.
The arrows indicate upregulation or downregulation of the genes when comparing the different stimuli. Statistical analysis was performed using unpaired T test with Welch’s correction.
| mRNA | 50 nM Vitamin D3 vs 1,000 Hz | 50 nM Vitamin D3 vs 50bnM Vitamin D3+1,000 Hz | 50 nM Vitamin D3+1,000 Hz vs 1,000 Hz | |||
|---|---|---|---|---|---|---|
| Adherent | Suspension | Adherent | Suspension | Adherent | Suspension | |
| CD14 | ↑ 559.5 Fold (p = 0.003) |
↑ 564.9 Fold (p = 0.016) |
No difference (p = 0.24) |
No difference (p = 0.41) |
↑ 466.0 Fold (p = 0.001) |
↑ 422.2 Fold (p = 0.036) |
| CD36 | ↑ 9.28 Fold (p = 0.004) |
↑ 2.55 Fold (p = 0.0003) |
No difference (p = 0.14) |
↑ 1.38 Fold (p = 0.008) |
↑ 6.73 Fold (p = 0.006) |
↑ 1.85 Fold (p = 0.002) |
| HLA-DRA | No difference (p = 0.57) |
↓ 0.15 Fold (p = 0.0001) |
No difference (p = 0.87) |
No difference (p = 0.14) |
No difference (p = 0.39) |
↓ 0.18 Fold (p < 0.0001) |
| HLA-DMB | No difference (p = 0.58) |
↑ 2.51 Fold (p = 0.0008) |
No difference (p = 0.09) |
↑ 1.43 Fold (p = 0.006) |
No difference (p = 0.61) |
↑ 1.75 Fold (p = 0.002) |
| NFAT2 | No difference (p = 0.075) |
No difference (p = 0.14) |
No difference (p = 0.28) |
No difference (p = 0.15) |
No difference (p = 0.052) |
↓ 0.84 Fold (p = 0.02) |
| TCF3 | ↓ 0.13 Fold (p = 0.031) |
↓ 0.58 Fold (p = 0.013) |
No difference (p = 0.44) |
↓ 0.77 Fold (p = 0.045) |
↓ 0.15 Fold (p = 0.009) |
No difference (p = 0.08) |
| TCF4 | ↓ 0.18 Fold (p = 0.032) |
↓ 0.54 Fold (p < 0.0001) |
No difference (p = 0.28) |
No difference (p = 0.13) |
↓ 0.14 Fold (p = 0.029) |
↓ 0.59 Fold (p < 0.0001) |
| TCF7L2 | No difference (p = 0.32) |
↑ 1.84 Fold (p = 0.0034) |
No difference (p = 0.63) |
No difference (p = 0.29) |
No difference (p = 0.46) |
↑ 2.09 Fold (p = 0.0047) |
| LEF-1 | n/a | ↓ 0.36 Fold (p = 0.0014) |
No difference (p = 0.3) |
↓ 0.56 Fold (p = 0.011) |
n/a | ↓ 0.65 Fold (p = 0.0027) |
| PKD1 | No difference (p = 0.2) |
No difference (p = 0.06) |
No difference (p = 0.15) |
No difference (p = 0.91) |
No difference (p = 0.54) |
No difference (p = 0.055) |
| PKD2 | ↑ 4.09 Fold (p = 0.0036) |
↑ 2.2 Fold (p < 0.0001) |
No difference (p = 0.16) |
No difference (p = 0.74) |
↑ 3.24 Fold (p = 0.0001) |
↑ 2.3 Fold (p = 0.0039) |
| PIEZO1 | ↓ 0.11 Fold (p = 0.026) |
↓ 0.59 Fold (p = 0.015) |
No difference (p = 0.42) |
No difference (p = 0.478) |
↓ 0.12 Fold (p = 0.027) |
No difference (p = 0.052) |