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. 2021 Jun 9;41(7):596–614. doi: 10.1002/cac2.12181

FIGURE 2.

FIGURE 2

LINC00525 overexpression promotes LUAD cell proliferation. A. RNAi (shLINC00525) treatment reduced the expression of LINC00525 in both A549 and PC9 cells. B. LINC00525 overexpression increased LINC00525 expression in both A549 and PC9 cells. C‐D. KEGG pathway enrichment (C), and GSEA analysis (D) showed that the deregulated genes following LINC00525 knockdown were associated with the cell cycle. E‐F. RTCA was used to detect proliferation of A549 cells. Knockdown of LINC00525 markedly impaired, whereas overexpression of LINC00525 promoted the proliferation of A549 cells. G. Knockdown and overexpression of LINC00525 suppressed and induced the proliferation of A549 cells, respectively, as measured by EdU assay. H. Knockdown and overexpression of LINC00525 suppressed and induced clonogenicity of A549 cells, respectively. I‐J. Flow cytometric analyses were performed to analyze cell cycle progression in A549 cells. Overexpression and knockdown of LINC00525 inhibited and facilitated G1 to S transition in A549 cells, respectively. *P < 0.05; **P < 0.01; ***P < 0.001. Error bars, SEM. Abbreviations: EdU, 5‐Ethynyl‐20‐deoxyuridine; GSEA, Gene Set Enrichment Analysis; LUAD, lung adenocarcinoma; qRT‐PCR, quantitative reverse transcription polymerase chain reaction; RTCA, real‐time xCELLLigence analysis system