FIGURE 6.

LINC00525 promotes cell proliferation by binding to EZH2 and RBMS2. A. Schematic diagram of the study design for the rescue experiments. B‐D. Knockdown of EZH2 and overexpression of RBMS2 abrogated the effects of LINC00525 on cell growth, as shown by RTCA assay (B), EdU (C), and colony formation (D) assays, respectively. E. p21 mRNA levels were determined by qRT‐PCR in A549 cells following the treatments indicated.. F. P21, cyclin D1, cyclin E1, CDK4 and CDK6 expression was evaluated by western blotting in A549 cells following the treatments indicated. *P < 0.05; **P < 0.01; ***P < 0.001. Error bars, SEM. Abbreviations: EdU, 5‐Ethynyl‐20‐deoxyuridine; qRT‐PCR, quantitative reverse transcription polymerase chain reaction; RTCA, real‐time xCELLLigence analysis system