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. 2021 May 15;41(7):560–575. doi: 10.1002/cac2.12158

FIGURE 4.

FIGURE 4

Overexpression of PKM2 reverses the inhibition of aerobic glycolysis and cell proliferation caused by ALYREF knockdown in BCa cells. (A) Western blotting of PKM2 expression in 5637 and T24 cells co‐transfected with lentivirus vector (ALYREF knockdown or control) and plasmid vector (PKM2 or control). (B) Glucose assay, lactic acid assay, and ATP assay showe that overexpression of PKM2 rescues inhibition of aerobic glycolysis caused by ALYREF knockdown in 5637 and T24 cells. (C) Overexpression of PKM2 rescues the cell proliferation affected by ALYREF knockdown in 5637 and T24 cells by CCK‐8 assays. (D) Overexpression of PKM2 rescues the colony formation affected by ALYREF knockdown in 5637 and T24 cells by colony formation assays. * P < 0.05, ** P < 0.01, *** P < 0.001. Abbreviations: PKM2, Pyruvate kinase muscle isozyme M2; GFP, green fluorescent protein; shNC, negative control of knockdown; ALYREF, Aly/REF export factor; shALYREF, knockdown of Aly/REF export factor; BCa, bladder cancer.