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. Author manuscript; available in PMC: 2022 Jul 15.
Published in final edited form as: Mol Cell. 2021 Jun 8;81(14):3007–3017.e5. doi: 10.1016/j.molcel.2021.05.014

Figure 2. RADX silencing protects stalled forks from degradation in the absence of BRCA2 without restoring RAD51 localization.

Figure 2.

(A and B) Fork protection assays were completed in (A) hTERT-RPE-1 or (B) DLD1 cells with and without BRCA2 after transfection of the indicated siRNAs. (C) Neutral comet assay in siRNA transfected U2OS cells treated with 3mM HU for 5 hours. (D) U2OS cells transfected with siRNA were labeled with 10μM EdU for 20 minutes, treated with 3mM HU for 5h, and stained for RAD51 and EdU. Representative images of RAD51 staining and the number of chromatin-bound RAD51 foci per nucleus is shown (mean +/− 95% confidence interval). Scale bar-10μm. A Mann-Whitney test was used to calculate p-values. See also Figure S3.