Skip to main content
. Author manuscript; available in PMC: 2022 Jul 15.
Published in final edited form as: Mol Cell. 2021 Jun 8;81(14):3007–3017.e5. doi: 10.1016/j.molcel.2021.05.014

Figure 4. RADX inactivation rescues fork reversal-dependent telomere instability only in cells treated with aphidicolin.

Figure 4.

(A) Schematic model indicating that fork reversal causes telomere catastrophe in RTEL1-deficient cells (Margalef et al., 2018). (B) Representative images of normal, fragile, and unequal telomeres. Scale bar-1μm. (C) Quantification of fragile telomeres and (D) telomere heterogeneity in cells transfected with siRNAs. (E) Quantification of fragile telomeres and (F) telomere heterogeneity in cells transfected with siRNAs and treated with 0.2μM aphidicolin for 40h. Each data point represents a metaphase spread. All experiments were completed at least three times. Parentheses indicate the number of total metaphases analyzed. P values were calculated using a Kruskal-Wallis test. See also Figure S4.