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. 2021 Jul 18;18:159. doi: 10.1186/s12974-021-02167-2

Fig. 1.

Fig. 1

Effects of central Ad-CTRP4 injection on hypothalamic CTRP4 protein expression, food intake, and body weight in mice. (a-b) At 72 h after i.c.v. injection, hypothalami were collected from mice fed a standard chow diet (SCD) or a high-fat diet (HFD). Representative Western blot (a) and quantitative analysis of CTRP4 expression (b) in the hypothalamus of mice that received saline (SCS and HFS), Ad-GFP (HFG), and Ad-CTRP4 (HFC). (c) Daily food intake after central intervention. (d) Changes in body weight after central intervention. The data are the mean ± SD. n = 5 per group. Two-way analysis of variance (ANOVA) and Tukey’s test for multiple comparisons were used to analyze differences among groups. #P<0.05, ##P<0.01 vs. SCS group, *P<0.05, **P<0.01 vs. HFG group