Fig. 5.

Effects of central CTRP4 overexpression on hypothalamic NF-κB signaling. At 72 h after i.c.v. injection, the mice receiving saline (SCS and HFS), Ad-GFP (HFG), and Ad-CTRP4 (HFC) were anesthetized and hypothalamic tissue was collected. (a, c, e, g, i) Representative immunoblots showing hypothalamic total p-P65 and P65 (a), nuclear and cytoplasmic P65 (c, e), p-IKKα/β and IKKα/β (g), and p-IκBα (i) in mice among groups. (b, d, f, h, j) Quantitative analysis of p-P65 (b), nuclear and cytoplasmic P65 (d, f), p-IKKα/β (h) and p-IκBα (j) protein. (k) Representative images of p-P65 immunoreactivity (red) and DAPI nuclear staining (blue) in the ARCs of mice among groups. (l) Quantitative analysis of the results in panel k. n = 5 per group. Two-way analysis of variance (ANOVA) and Tukey’s test for multiple comparisons were used to analyze differences among groups. ^#P<0.05, ^##P<0.01 vs. SCS group, *P<0.05, **P<0.01 vs. HFG group