Fig. 1.

Genetic deletion of Il10 promotes tau hyperphosphorylation after LPS injection. Non-Tg or Il10^−/− mice injected with LPS or Veh were sacrificed 24 h after LPS injection and brain tissue was extracted for analysis. A Western immunoblot (WB) of hippocampal (Hp) homogenates to assess levels of phosphorylated AT8, AT180, and PHF-1 tau epitopes. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was analyzed as a protein loading control. B–D Quantification of immunoreactive bands as a ratio of pTau (AT180, AT8, and PHF-1) over total tau (Tau5). E Quantification of immunoreactive bands as a ratio of Tau5 over GAPDH. Data shown are mean + SEM of integrated density value (IDV) ratio; * p < 0.05, ** p < 0.01, *** p < 0.001; two-way ANOVA with Sidak’s multiple comparison test; n = 10–18 mice; each individual data point shows an individual mouse sample