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. 2021 Jul 18;18:161. doi: 10.1186/s12974-021-02211-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — IL-6 stimulation increased p-tau in primary mouse neurons, in vitro. A WB of cell lysates from primary neuronal cell cultures directly treated with recombinant IL-6 protein (25 ng/mL) or Veh. B Quantification of pTau over total tau, AT8/Tau5, and PHF-1/Tau5 ratios. C Quantification of pTau (AT8/GAPDH and PHF-1/GAPDH) and total tau (Tau5/GAPDH) relative to loading control. D Quantification of (activated) phospho-STAT3 (P-STAT3) relative to (total) STAT3. Quantification of P-STAT3 and STAT3 relative to GAPDH. E, F WB of cell lysates of adult neurons from non-transgenic (Non-Tg) and IL-10-deficient mice (Il10^−/−) show significantly higher PHF-1/Tau5 ratio in IL-10-deficient neurons treated with 25 ng/mL recombinant IL-6 compared to Non-Tg neurons treated with the same dose of IL-6. Data shown are mean ± SEM of IDV ratio; * p < 0.05, **p < 0.01; *** p < 0.001; unpaired t tests; n = 3 neuronal culture wells/treatment group