Figure 3.

Th1 inflammation is not evident in the adipose tissue of cancer bearing mice. (A–B) Gene set enrichment plots depicting the upregulation of both IFNγ (A) and IFNα/β (B) signaling pathways in VAT of cancer bearing mice. (C) Heatmap depicting sample-level expression of genes in the IFNγ signaling pathway which are most strongly upregulated in the VAT of cancer bearing mice. (D–H) Cytokine array quantifications of Th1-related cytokines including IFNγ (D), TNFα (E), IL-2 (F), CXCL9 (G), and CXCL10 (H). (I-J) Quantification of cell types by flow cytometry, including CD8⁺ T cells (I) and CD11c⁺ dendritic cells (J). Statistical analyses were performed using the Mann–Whitney test; mean ± SD is shown.