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. 2021 Jul 2;478(13):2425–2443. doi: 10.1042/BCJ20210200

Figure 2. Activity of different RdRp preparations in the strand displacement assay.

Figure 2.

RdRp was mixed at the indicated concentrations with 100 nM RNA substrate and 300 µM of each NTP and Cy3 fluorescence was recorded. Enzyme preincubation was performed where indicated at 22°C for 30 min at the indicated ratios (5 µM nsp12). (A) Sf nsp12-F/7H8. (B) Sf nsp12-HF/7L8. (C) Sf nsp12-HF after preincubation with Sf 7H8 (1 : 3 ratio). (D) Ec nsp12 after preincubation with Ec nsp7 and Ec nsp8 (1 : 3 : 6 ratio). (E) Ec nsp12 after preincubation with Sf 7H8 (1 : 3 ratio). (F) Sf nsp12-HF after preincubation with Ec nsp7 and Ec nsp8 (1 : 3 : 6 ratio).